Diversity of chemical composition and nutritional value in grain from selected winter wheat cultivars grown in south-western Poland.

Given the low protein coverage by legumes in Poland, alternatives (with high protein content and high nutritional value) are being sought (with high protein content and high nutritional value of protein) that could replace these plants. Cereal cultivation dominates in Poland; hence, the search for high-value plants will also consider this group of plants. The aim of the study was to compare the nutritional value of proteins from two wheat cultivars. A field experiment conducted in Zawidowice in south-western Poland in 2019 investigated the nutritional values of two winter wheat cultivars: Aurelius and Activus. These two cultivars were compared in terms of their chemical composition, the biological value of their proteins for animal nutrition, and the content of macro- and microelements. Significant differences in chemical composition were found between the tested wheat cultivars. In terms of the chemical composition, i.e. the content of protein, fiber and ash, the Activus cultivar was characterized by significantly better parameters. This cultivar also had significantly higher gross energy. In turn, a significantly higher content of essential amino acids, i.e. lysine, cysteine, tryptophan, histidine, leucine, ioleucine, and valine, was found in the Aurelius cultivar; therefore, the indicators determining the biological value of the protein are more favorable in the Aurelius cultivars. Meanwhile, in terms of selected macro- and microelements the Auerlius cultivar was more valuable. Varietal progress is necessary to obtain cultivars with the essential nutrients needed by animals to satisfy their dietary requirements.

The impact of the distribution method for struvite (Crystal Green) on the chemical composition of soybean and their utility in animal nutrition.

One of the main factors considered in assessing the nutritional value of feed is its chemical composition, which can be modified by fertilization. Faced with reducing P resources, alternative sources of this element are being sought. Phosphorus is an essential nutrient in soybean cultivation. The aim of the study was to use an alternative source of phosphorus fertilizer and compare its impact on the chemical composition of soybean seeds with that of a traditional fertilizer (Super FOS DAR). The study investigated a range of factors in animal nutrition as well as the basic content of macro- and microelements. A pot experiment with the Abelina soybean variety was conducted at the Experimental Station of the Wroclaw University of Environmental and Life Sciences. The experiment considered two factors against the control: phosphorus fertilizer placement (band, broadcast) and different phosphorus fertilization (Super FOS DAR, Crystal Green). Use of struvite (Crystal Green)) caused positive changes in selected amino acids content and in the nutritional value of protein in soybean seeds; this can enhance the value of soybean seeds as well as increase certain macroelements and microelements. Phosphorus fertilizer significantly increased the content of lysine, leucine, valine, phenyloalanine and tyrosine. Band fertilization with struvite caused a significant increase in amino acids (lysine, leucine, valine, phenyloalanine and tyrosine) as well as in the nutritional value of protein (as measured by the essential amino acid index, protein efficiency ratio and biological value of the protein). Favorable changes under the influence of the application of struvite were recorded in the content of calcium, as well as phosphorus, iron, and manganese. The value of the struvite in the case of its use as phosphorus fertilizer is promising; however, it needs further study.

Effect of struvite (Crystal Green) fertilization on soil element content determined by different methods under soybean cultivation.

Struvite is regarded as a promising phosphorus fertilizer alternative to mineral fertilizers; however before fertilizing, soil tests should be undertaken to determine fertilizer recommendations. In May 2022, soil was sampled from a pot experiment with the application of phosphorus set up at the Wroclaw University and Environmental and Life Sciences. Chemical analysis of the soil included total and available phosphorus, potassium, magnesium determined by the Egner-Riehm, Mehlich 3 and Yanai methods. The purpose of the article is to compare soil element extraction by three different methods under struvite fertilization and its use in soybean cultivation. The application of these methods indicated an unambiguous increase in soil Mg content after struvite application. Broadcast soybean fertilization affected the phosphorus content of the soil. The results of the study indicated that different extraction methods presented different contents of P from soil. The content of available phosphorus was circa 122-156 mg kg-1 dm, 35.4-67.5 mg kg-1 dm and 100-159 mg kg-1 dm according to the Mehlich, Yanai and Egner-Riehm methods, respectively. A positive correlation was found between the content of Mg and K in soil determined by the Mehlich 3 and Yanai methods, which may suggest that the Yanai method could be introduced into standard soil chemical analysis in Poland. Such a correlation was not found for phosphorus, which is a difficult element to determine due to the multitude of factors affecting its availability.

Removal of selected pollutants from landfill leachate in constructed wetlands with different filling.

Constructed wetlands (CW) with vertical flow were used to treat leachate from municipal landfills (active and closed, at different concentrations) using a combination of substrates, i.e., gravel, sand and an exchangeable layer, depending on a variant: organic substrate (pine bark) or mineral substrate (zeolite, expanded clay). The systems were planted with Phragmites australis. The aim of this study was to compare the efficiency of removal of selected pollutants from landfill leachate in CW using different types of filling. For most parameters, the best reductions were obtained on zeolite substrates. In the investigated CW, reductions were achieved at the levels of: AN 96%-99%; TKN 75%-88.5%; TN 62.5%-70%, TP 84-88% and heavy metals (Zn, Ni, Cu, Cr) 41%-56%.

Assessment and monitoring of soil and plant contamination with trace elements around Europe's largest copper ore tailings impoundment.

Europe's largest copper ore tailings impoundment has been considered a potential source of risk for human health, thus leading to the elimination of agricultural production in the surrounding area and its subsequent afforestation. The aim of this study was to analyse the level, spatial distribution and temporal changes in soil and edible plant contamination with trace elements around the impoundment, taking into account the local soil properties. The mean concentrations of Zn, Pb and As (31.5, 19.3 and 3.9 mg kg-1, respectively) were found to be higher than median values in soils of Poland, but they do not indicate soil pollution or enrichment when assessed using the relative geochemical index (Igeo) and enrichment factor (EF). The Cu concentration (mean 25.6 mg kg-1) was significantly higher than the median value for Polish soils, indicating moderate to high pollution/enrichment. A relationship between Cu concentration in topsoil and distance to the impoundment, in particular in its eastern forefield, indicates that this landfill site may be considered the source of soil contamination with Cu. However, both the mean and maximum concentrations of all elements under study, including Cu, were below the legal intervention levels. Long-term topsoil monitoring, although high data variability on permanent plots, has documented stable concentration or slow decrease of element concentration over a period 1995-2016. Cu and Zn concentrations in vegetables from home gardens do not differ from typical values in commercially available products, whereas higher than typical concentrations of Pb and As may result from other local sources of contamination. Low level and stable soil and plant contamination with trace metals justifies continuation of crop production and no need for the further conversion of arable lands into forests. The relatively little negative impact of the tailings impoundment, despite its large dimensions, results probably from implemented effective anti-emission measures.

The Release of Antimony from Mine Dump Soils in the Presence and Absence of Forest Litter.

This study examined the changes in antimony (Sb) solubility in soils, using organic matter introduced with forest litter, in various moisture conditions. Soils containing 12.8⁻163 mg/kg Sb were taken from the top layers of dumps in former mining sites in the Sudetes, South-West Poland. Soils were incubated for 90 days either in oxic or waterlogged conditions, with and without the addition of 50 g/kg of beech forest litter (FL). Water concentrations of Sb in some experimental treatments greatly exceeded the threshold values for good quality underground water and drinking water, and reached a maximum of 2.8 mg/L. The changes of Sb solubility caused by application of FL and prolonged waterlogging were, in various soils, highly divergent and in fact unpredictable based on the main soil properties. In some soils, the application of forest litter prompted the release of Sb from soil solid phase, while in the others it acted contradictorily. Soil waterlogging resulted, in most cases, in the increased release of Sb compared to oxic conditions, and this effect was enhanced by the addition of forest litter. However, in two soils the presence of forest litter counteracted the effects of waterlogging and diminished the quantities of released Sb.

Seasonal dynamics of nitrate and ammonium ion concentrations in soil solutions collected using MacroRhizon suction cups.

The aims of the study were to analyse the concentration of nitrate and ammonium ions in soil solutions obtained using MacroRhizon miniaturized composite suction cups under field conditions and to determine potential nitrogen leaching from soil fertilized with three types of fertilizers (standard urea, slow-release urea, and ammonium nitrate) at the doses of 90 and 180 kg ha-1, applied once or divided into two rates. During a 3-year growing experiment with sugar sorghum, the concentration of nitrate and ammonium ions in soil solutions was the highest with standard urea fertilization and the lowest in variants fertilized with slow-release urea for most of the months of the growing season. Higher concentrations of both nitrogen forms were noted at the fertilizer dose of 180 kg ha-1. One-time fertilization, at both doses, resulted in higher nitrate concentrations in June and July, while dividing the dose into two rates resulted in higher nitrate concentrations between August and November. The highest potential for nitrate leaching during the growing season was in July. The tests confirmed that the miniaturized suction cups MacroRhizon are highly useful for routine monitoring the concentration of nitrate and ammonium ions in soil solutions under field conditions.

Some adverse effects of soil amendment with organic Materials-The case of soils polluted by copper industry phytostabilized with red fescue.

The study was aimed to examine the effects of soil amendment with organic waste materials on the growth of red fescue and the uptake of Cu and Zn by this grass, in view of its potential usage for phytostabilization of Cu-polluted soils. Five soils, containing 301-5180 mg/kg Cu, were collected from the surroundings of copper smelter Legnica, and amended with lignite (LG) and limed sewage sludge (SS). Plant growth and the concentrations of Cu and Zn in the shoots and roots of grass were measured in a pot experiment and related to the results of Pytotoxkit and Microtox® tests performed on soil solution. The effects of soil amendment with LG and SS differed greatly, and depended on soil properties. In some cases, the application of alkaline SS resulted in dramatic increase of Cu phytotoxicity and its enhanced uptake by plants, while application of LG to slightly acidic soil caused increased accumulation of Zn in plants, particularly in their roots. The study confirmed good suitability of red fescue for phytostabilization of Cu-contaminated soils except for those extremely polluted. Organic amendments to be used for metal immobilization should be thoroughly examined prior to application.

Arsenic extractability and uptake by velvetgrass Holcus lanatus and ryegrass Lolium perenne in variously treated soils polluted by tailing spills.

Phytostabilization should be considered as an appropriate phytoremediation technique to restore the area affected by tailing spills in Zloty Stok, where arsenic ores were mined and processed for several centuries. The study aimed to compare the suitability of velvetgrass (Holcus lanatus L.) and ryegrass (Lolium perenne L.) for development of plant cover in that area. Various treatments commonly applied to support phytostabilization were examined. A pot experiment was carried out to assess the effects of soil amendment with phosphate (P), sewage sludge (SS) and iron salts (Fe) on arsenic extractability and its uptake by grass. Four kinds of soil material, containing 356-5350 mg kg(-1) As, were examined. Velvetgrass proved to be more resistant than ryegrass to the toxicity of soil arsenic. Ammonium sulphate extractability of As in soils correlated well with As concentrations in the biomass of both grass species. In three of four tested soils, application of Fe failed to decrease As extractability and to reduce its concentrations in the aboveground parts of grasses. Application of P and SS resulted in increased As solubility in soils, but their effects on plant biomass and As uptake were ambiguous. SS had a strong beneficial influence on the growth of velvetgrass, while such an effect was not observed for ryegrass.